UNITED STATES
SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

FORM N-Q

QUARTERLY SCHEDULE OF PORTFOLIO HOLDINGS OF REGISTERED
MANAGEMENT INVESTMENT COMPANIES

Investment Company Act file number 811- 21131

John Hancock Preferred Income Fund
(Exact name of registrant as specified in charter)

601 Congress Street, Boston, Massachusetts 02210
(Address of principal executive offices) (Zip code)

Alfred E. Ouellette, Senior Counsel and Assistant Secretary

601 Congress Street

Boston, Massachusetts 02210

(Name and address of agent for service)

Registrant's telephone number, including area code: 617-663-4324

Date of fiscal year end:  July 31 
 
  
Date of reporting period:  October 31, 2007 

ITEM 1. SCHEDULE OF INVESTMENTS




John Hancock
Preferred Income Fund
Securities owned by the Fund on
October 31, 2007 (unaudited)

  Interest  Maturity  Credit  Par value   
Issuer, description  rate  date  rating (A)  (000)  Value 

Bonds 3.30%          $19,396,764 
(Cost $19,771,543)           
 
Electric Utilities 1.81%          10,612,489 
Black Hills Corp.,           
Note  6.500%  05-15-13  BBB-  $5,950  5,946,269 
Entergy Gulf States, Inc.,           
1st Mtg Bond  6.200  07-01-33  BBB+  5,000  4,666,220 
Gas Utilities 1.49%          8,784,275 
Southern Union Co.,           
Jr Sub Note Ser A  7.200  11-01-66  BB  8,800  8,784,275 
      
        Interest  Maturity  Credit  Par value   
Issuer, description  rate  date  rating (A)  (000)  Value 

Capital preferred securities 10.06%          $59,119,786 
(Cost $55,483,024)           
 
Diversified Banks 1.25%          7,331,250 
Lloyds TSB Bank Plc, (United Kingdom) (F)  6.90%  11-29-49  A+  $7,500  7,331,250 
Electric Utilities 4.66%          27,385,824 
DPL Capital Trust II  8.125  09-01-31  BB+  24,000  27,385,824 
Gas Utilities 4.15%          24,402,712 
KN Capital Trust I, Ser B  8.56  04-15-27  B-  17,500  17,150,000 
KN Capital Trust III  7.63  04-15-28  B-  8,000  7,252,712 
   
Issuer        Shares  Value 

Common stocks 3.59%          $21,097,929 
(Cost $19,502,471)           
 
Electric Utilities 0.69%          4,028,400 
Great Plains Energy, Inc.        135,000  4,028,400 
Integrated Oil & Gas 0.49%          2,905,829 
BP Plc, ADR (United Kingdom) (F)        37,259  2,905,829 
Multi-Utilities 2.41%          14,163,700 
Alliant Energy Corp.        190,000  7,600,000 
TECO Energy, Inc.        390,000  6,563,700 

Page 1


John Hancock
Preferred Income Fund
Securities owned by the Fund on
October 31, 2007 (unaudited)

  Credit     
  rating (A)  Shares  Value 

Preferred stocks  127.41%      $748,720,647 
       
 
Agricultural Products  2.17%      12,749,351 
Ocean Spray Cranberries, Inc., 6.25%,       
  BB+  143,000  12,749,351 
 
Automobile Manufacturers 2.90%      17,044,312 
  CCC+  40,000  743,200 
General Motors Corp., 7.25%, Ser 04-15-41  B-  378,700  6,888,553 
General Motors Corp., 7.25%, Ser 07-15-41  B-  82,000  1,506,340 
General Motors Corp., 7.25%, Ser 02-15-52  B-  254,300  4,585,029 
General Motors Corp., 7.375%,       
  B-  50,000  920,500 
General Motors Corp., 7.375%,       
  B-  129,000  2,400,690 
 
Broadcasting & Cable TV 4.39%      25,777,582 
  BBB  188,600  4,445,302 
  BBB+  232,000  5,758,240 
Comcast Corp., 7.00%, Ser B  BBB+  629,000  15,574,040 
 
Consumer Finance 6.25%      36,750,429 
Ford Motor Credit Co., 7.60%  B1  308,500  6,185,425 
HSBC Finance Corp., 6.00%  AA-  134,200  3,093,310 
HSBC Finance Corp., 6.36%, Depositary       
  A  297,000  7,303,230 
HSBC Finance Corp., 6.875%  AA-  404,800  10,140,240 
  BBB+  194,100  3,447,216 
SLM Corp., 6.97%, Ser A  BBB-  147,391  6,581,008 

Page 2


John Hancock
Preferred Income Fund
Securities owned by the Fund on
October 31, 2007 (unaudited)

Diversified Banks 8.20%      48,216,900 
BAC Capital Trust IV, 5.875%  A+  30,000  654,900 
Bank One Capital Trust VI, 7.20%  A  81,100  2,027,500 
Barclays Bank Plc, 7.10%, Ser 3       
(United Kingdom) (F)  A+  50,000  1,265,000 
Fleet Capital Trust VIII, 7.20%  A+  484,250  12,115,935 
HSBC Holdings Plc, 6.20%, Ser A       
(United Kingdom) (F)  A  161,000  3,625,720 
Royal Bank of Scotland Group Plc, 5.75%,       
Ser L (United Kingdom) (F)  A  550,900  11,607,463 
Royal Bank of Scotland Group Plc, 6.35%,       
Ser N, ADR (United Kingdom) (F)  A  25,000  575,500 
Royal Bank of Scotland Group Plc, 7.25%,       
Ser T (United Kingdom) (F)  A  59,000  1,502,730 
Santander Finance Preferred SA,       
Unipersonal, 6.41%, Ser 1 (Spain) (F)  A+  225,000  5,220,000 
USB Capital VIII, 6.35%, Ser 1  A+  179,800  4,077,864 
USB Capital X, 6.50%  A+  45,000  1,045,350 
Wachovia Preferred Funding Corp., 7.25%,       
Ser A  A  69,000  1,787,790 
Wells Fargo Capital Trust IV, 7.00%  AA-  108,100  2,711,148 
 
Electric Utilities 25.16%      147,850,485 
Duquesne Light Co., 6.50%  BB  73,650  3,744,646 
Entergy Arkansas, Inc., 6.70%  AAA  25,300  640,849 
Entergy Mississippi, Inc., 7.25%  A-  363,900  9,170,280 
FPC Capital I, 7.10%, Ser A  BBB-  860,691  21,560,310 
FPL Group Capital Trust I, 5.875%  BBB+  502,200  11,399,940 
FPL Group Capital, Inc., 7.45%, Ser E  BBB+  110,000  2,854,500 
Georgia Power Capital Trust VII, 5.875%  BBB+  250,600  5,535,754 
Georgia Power Co., 6.00%, Ser R  A  415,497  10,079,957 
HECO Capital Trust III, 6.50%  BB+  375,400  8,919,504 
Interstate Power & Light Co., 8.375%,       
Ser B  Baa2  700,000  21,070,000 
NSTAR Electric Co., 4.78%  A-  15,143  1,309,869 
PPL Electric Utilities Corp., 6.25%,       
Depositary Shares  BBB  300,000  7,678,140 
PPL Energy Supply, LLC, 7.00%  BBB  563,160  14,163,474 
Southern California Edison Co., 6.125%  BBB-  119,000  12,390,875 
Virginia Power Capital Trust, 7.375%  BB+  596,250  14,924,137 
Westar Energy, Inc., 6.10%  AAA  97,500  2,408,250 
 
Gas Utilities 3.14%      18,451,038 
Southern Union Co., 5.00%, Conv  BBB-  10,000  565,000 
Southern Union Co., 7.55%, Ser A  BB  296,600  7,530,674 
Southwest Gas Capital II, 7.70%  BB  412,400  10,355,364 

Page 3


John Hancock
Preferred Income Fund
Securities owned by the Fund on
October 31, 2007 (unaudited)

Integrated Telecommunication Services 5.25%      30,870,378 
AT&T, Inc., 6.375%  A  66,000  1,589,940 
Telephone & Data Systems, Inc., 6.625%  BB+  233,000  4,694,950 
Telephone & Data Systems, Inc., 7.60%,       
Ser A  BB+  815,687  19,258,370 
Verizon New England, Inc., 7.00%, Ser B  A3  209,317  5,327,118 
 
Investment Banking & Brokerage 10.87%      63,849,881 
Bear Stearns Capital Trust III, 7.80%  BBB+  53,600  1,341,608 
Goldman Sachs Group, Inc., 6.20%, Ser B  A  460,000  11,058,400 
Lehman Brothers Holdings Capital Trust III,       
6.375%, Ser K  A-  150,000  3,384,000 
Lehman Brothers Holdings, Inc., 5.94%,       
Depositary Shares, Ser C  A-  175,600  8,077,600 
Merrill Lynch Preferred Capital Trust III,       
7.00%  A-  366,400  8,921,840 
Merrill Lynch Preferred Capital Trust IV,       
7.12%  A-  276,552  6,781,055 
Merrill Lynch Preferred Capital Trust V,       
7.28%  A-  367,000  9,108,940 
Morgan Stanley Capital Trust III, 6.25%  A  192,400  4,313,608 
Morgan Stanley Capital Trust IV, 6.25%  A  57,000  1,270,530 
Morgan Stanley Capital Trust V, 5.75%  A1  347,000  7,252,300 
Morgan Stanley Capital Trust VI, 6.60%  A  100,000  2,340,000 
 
Life & Health Insurance 7.08%      41,580,962 
Lincoln National Capital VI, 6.75%, Ser F  A-  175,800  4,264,908 
MetLife, Inc., 6.50%, Ser B  BBB  950,500  23,629,430 
PLC Capital Trust IV, 7.25%  BBB+  208,300  5,182,504 
PLC Capital Trust V, 6.125%  BBB+  256,000  5,637,120 
Prudential Plc, 6.50%       
(United Kingdom) (F)  A-  122,000  2,867,000 
 
Movies & Entertainment 4.06%      23,877,976 
Viacom, Inc., 6.85%  BBB  985,065  23,877,976 
 
Multi-Line Insurance 7.17%      42,114,431 
Aegon NV, 6.375% (Netherlands) (F)  A-  444,900  10,223,802 
Aegon NV, 6.50% (Netherlands) (F)  A-  111,100  2,565,299 
ING Groep NV 6.125% (Netherlands) (F)  A  61,500  1,349,925 
ING Groep NV, 6.20% (Netherlands) (F)  A  156,993  3,477,395 
ING Groep NV, 7.05% (Netherlands) (F)  A  760,100  18,926,490 
ING Groep NV, 7.20% (Netherlands) (F)  A  100,000  2,506,000 
ING Groep NV, 7.375% (Netherlands) (F)  A  120,500  3,065,520 

Page 4


John Hancock

Preferred Income Fund

Securities owned by the Fund on October 31, 2007 (unaudited)

Multi-Utilities 7.72%      45,377,868 
Baltimore Gas & Electric Co., 6.99%,       
Ser 1995  Ba1  40,000  4,125,000 
BGE Capital Trust II, 6.20%  BBB-  836,825  18,544,042 
DTE Energy Trust I, 7.80%  BB+  136,400  3,422,276 
PNM Resources, Inc., 6.75%, Conv  BBB-  284,500  12,785,430 
PSEG Funding Trust II, 8.75%  BB+  233,500  5,888,870 
Public Service Electric & Gas Co.,       
4.18%, Ser B  BB+  7,900  612,250 
 
Oil & Gas Exploration & Production 5.54%      32,578,271 
Chesapeake Energy Corp., 6.25%, Conv (G)  B+  4,530  1,431,571 
Nexen, Inc., 7.35% (Canada) (F)  BB+  1,261,000  31,146,700 
 
Other Diversified Financial Services 15.56%      91,444,548 
ABN AMRO Capital Funding Trust V, 5.90%  A  626,100  13,354,713 
ABN AMRO Capital Funding Trust VII, 6.08%  A  328,000  7,137,280 
Citigroup Capital VII, 7.125%  A+  336,500  8,419,230 
Citigroup Capital VIII, 6.95%  A+  648,100  15,923,817 
Citigroup Capital X, 6.10%  A+  40,000  901,200 
Citigroup Capital XI, 6.00%  A+  25,000  551,750 
DB Capital Funding VIII, 6.375%  A+  471,250  10,942,425 
DB Capital Trust II, 6.55%  A+  526,750  12,615,662 
JPMorgan Chase Capital X, 7.00%, Ser J  A  615,100  15,346,745 
JPMorgan Chase Capital XI, 5.875%, Ser K  A  289,700  6,251,726 
 
Real Estate Management & Development 3.68%      21,639,540 
Duke Realty Corp., 6.50%, Depositary       
Shares, Ser K  BBB  110,000  2,442,000 
Duke Realty Corp., 6.60%, Depositary       
Shares, Ser L  BBB  109,840  2,452,727 
Duke Realty Corp., 6.625%, Depositary       
Shares, Ser J  BBB  59,925  1,350,110 
Public Storage, Inc., 6.45%, Depositary       
Shares, Ser X  BBB+  25,000  544,500 
Public Storage, Inc., 6.50%, Depositary       
Shares, Ser W  BBB+  100,000  2,225,000 
Public Storage, Inc., 7.50%, Depositary       
Shares, Ser V  BBB+  497,643  12,625,203 
 
Regional Banks 3.61%      21,221,799 
KeyCorp Capital VI, 6.125%  BBB  25,900  550,634 
PFGI Capital Corp., 7.75%  A  796,000  20,671,165 
 
Reinsurance 0.52%      3,031,380 
RenaissanceRe Holdings Ltd., 6.08%,       
Ser C (Bermuda) (F)  BBB  153,100  3,031,380 

Page 5


John Hancock
Preferred Income Fund
Securities owned by the Fund on
October 31, 2007 (unaudited)

Specialized Finance 1.05%      6,183,850 
CIT Group, Inc., 6.35%, Ser A  BBB+  145,000  3,233,500 
Repsol International Capital Ltd.,       
7.45%, Ser A (Cayman Islands) (F)  BB+  115,700  2,950,350 
Thrifts & Mortgage Finance 0.77%      4,503,400 
Sovereign Capital Trust V, 7.75%  BB+  178  4,503,400 
Wireless Telecommunication Services  2.32%      13,606,266 
United States Cellular Corp., 7.50%  BB+  582,460  13,606,266 

    Number of  Exercise  Expiration   
Issuer    Contracts  Price  Date  Value 

Purchased options  0.03%        $198,360 
(Cost $183,541)           
 
Options - Puts 0.03%          198,360 
iShares S&P SmallCap Index Fund  1,102  $88  March 08  198,360 
  Interest  Maturity  Credit  Par value   
Issuer, description  rate  date  rating (A)  (000)  Value 

Short-term investments 2.45%        $14,400,000 
(Cost $14,400,000)           
 
Government U.S. Agency  2.45%        14,400,000 
Federal Home Loan Bank,           
Discount Note  4.40% (Y)  11-01-07  AAA  $14,400  14,400,000 
 
Total investments (Cost $896,708,563)  146.84%      $862,933,486 
 
Other assets and liabilities, net 0.82%        $4,814,220 
 
Fund preferred shares, at liquidation value (47.66%)    ($280,093,084) 
 
Total net assets applicable to common shareholders  100.00%  $587,654,622 

The percentage shown for each investment category is the total value of that category, as a percentage of the net assets applicable to common shareholders.

Page 6


John Hancock
Preferred Income Fund
Notes to Schedule of Investments
October 31, 2007 (unaudited)

ADR American Depositary Receipt

(A) Credit ratings are unaudited and are rated by Moody's Investors Service or Fitch where Standard & Poor's ratings are not available unless indicated otherwise.

(F) Parenthetical disclosure of a foreign country in the security description represents country of a foreign issuer; however, security is U.S. dollar-denominated.

(G) Security rated internally by John Hancock Advisers, LLC.

(S) This security is exempt from registration under Rule 144A of the Securities Act of 1933. Such securities may be resold, normally to qualified institutional buyers, in transactions exempt from registration. Rule 144A securities amounted to $12,749,351 or 2.17% of the net assets applicable to common shareholders as of October 31, 2007.

(Y) Represents current yield as of October 31, 2007.

The cost of investments owned on October 31, 2007 , including short-term investments, was $896,708,563. Gross unrealized appreciation and depreciation of investments aggregated $17,402,549 and $51,177,626, respectively, resulting in net unrealized depreciation of $33,775,077.

Notes to Schedule of Investments - Page 1


John Hancock
Preferred Income Fund
Financial futures contracts
October 31, 2007 (unaudited)

  Number of      Unrealized 
Open contracts  contracts  Position  Expiration  Appreciation 

 
U.S. 10-year Treasury Note  840  Short  Dec-07  $466,616 

Financial futures contracts


John Hancock
Preferred Income Fund
Interest rate swap contracts
October 31, 2007 (unaudited)

  Rate type       

Notional  Payments made  Payments received  Termination    Unrealized 
amount  by Fund  by Fund  date  Counterparty  Depreciation 

 
$70,000,000  2.558% (a)  3-month LIBOR  Jun-08  Morgan Stanley  $976,560 

(a) Fixed rate

Interest rate swap contracts


Notes to portfolio of investments

Security valuation

The net asset value of the common shares of the Fund is determined daily as of the close of the New York Stock Exchange (NYSE), normally at 4:00 P.M., Eastern Time. Short-term debt investments that have a remaining maturity of 60 days or less are valued at amortized cost, and thereafter assume a constant amortization to maturity of any discount or premium, which approximates market value. All other securities held by the Fund are valued at the last sale price or official closing price (closing bid price or last evaluated quote if no sale has occurred) as of the close of business on the principal securities exchange (domestic or foreign) on which they trade or, lacking any sales, at the closing bid price. Securities traded only in the over-the-counter market are valued at the last bid price quoted by brokers making markets in the securities at the close of trading. Securities for which there are no such quotations, principally debt securities, are valued based on the valuation provided by an independent pricing service, which utilizes both dealer-supplied and electronic data processing techniques, which take into account factors such as institutional-size trading in similar groups of securities, yield, quality, coupon rate, maturity, type of issue, trading characteristics and other market data.

Other assets and securities for which no such quotations are readily available are valued at fair value as determined in good faith under consistently applied procedures established by and under the general supervision of the Board of Trustees. Generally, trading in non-U.S. securities is substantially completed each day at various times prior to the close of trading on the NYSE. The values of such securities used in computing the net asset value of the Fund's shares are generally determined as of such times. Occasionally, significant events that affect the values of such securities may occur between the times at which such values are generally determined and the close of the NYSE. Upon such an occurrence, these securities will be valued at fair value as determined in good faith under consistently applied procedures established by and under the general supervision of the Board of Trustees.

Futures

The Fund may purchase and sell financial futures contracts and options on those contracts. The Fund invests in contracts based on financial instruments such as U.S. Treasury Bonds or Notes or on securities indices such as the Standard & Poor's 500 Index, in order to hedge against a decline in the value of securities owned by the Fund.

Initial margin deposits required upon entering into futures contracts are satisfied by the delivery of specific securities or cash as collateral to the broker (the Funds' agent in acquiring the futures position). If the position is closed out by taking an opposite position prior to the settlement date of the futures contract, a final determination of variation margin is made, cash is required to be paid to or released by the broker and the Fund realizes a gain or loss.

When the Fund sells a futures contract based on a financial instrument, the Fund becomes obligated to deliver that kind of instrument at an agreed upon date for a specified price. The Fund realizes a gain or loss depending on whether the price of an offsetting purchase is less or more than the price of the initial sale or on whether the price of an offsetting sale is more or less than the price of the initial purchase. The Fund could be exposed to risks if it could not close out futures positions because of an illiquid secondary market or the inability of counterparties to meet the terms of their contracts. Futures contracts are valued at the quoted daily settlement prices established by the exchange on which they trade.

Notes to Schedule of Investments - Page 2


Swap contracts

The Fund may enter into swap transactions in order to hedge the value of the Fund's portfolio against interest rate fluctuations or to enhance the Fund's income. Interest rate swaps represent an agreement between two counterparties to exchange cash flows based on the difference in the two interest rates, applied to the notional principal amount for a specified period. The payment flows are usually netted against each other, with the difference being paid by one party to the other. The Fund settles accrued net receivable or payable under the swap contracts on a periodic basis.

The Fund records changes in the value of the swaps as unrealized gains or losses on swap contracts. Net periodic payments accrued, but not yet received (paid) are included in change in the unrealized appreciation/ depreciation on the Statement of Operations.

Swap contracts are subject to risks related to the counterparty's ability to perform under the contract, and may decline in value if the counterparty's creditworthiness deteriorates. The risks may arise from unanticipated movement in interest rates. The Fund may also suffer losses if it is unable to terminate outstanding swap contracts or reduce its exposure through offsetting transactions.

Notes to Schedule of Investments - Page 3




ITEM 2. CONTROLS AND PROCEDURES.

(a) Based upon their evaluation of the registrant's disclosure controls and procedures as conducted within 90 days of the filing date of this Form N-Q, the registrant's principal executive officer and principal accounting officer have concluded that those disclosure controls and procedures provide reasonable assurance that the material information required to be disclosed by the registrant on this report is recorded, processed, summarized and reported within the time periods specified in the Securities and Exchange Commission's rules and forms.

(b) There were no changes in the registrant's internal control over financial reporting that occurred during the registrant's last fiscal quarter that has materially affected, or is reasonably likely to materially affect, the registrant's internal control over financial reporting.

ITEM 3. EXHIBITS.

Separate certifications for the registrant's principal executive officer and principal accounting officer, as required by Rule 30a-2(a) under the Investment Company Act of 1940, are attached.


SIGNATURES

Pursuant to the requirements of the Securities Exchange Act of 1934 and the Investment Company Act of 1940, the registrant has duly caused this report to be signed on its behalf by the undersigned, thereunto duly authorized.

John Hancock Preferred Income Fund

By: /s/ Keith F. Hartstein
-------------------------------------
Keith F. Hartstein
President and
Chief Executive Officer

Date: December 19, 2007

Pursuant to the requirements of the Securities Exchange Act of 1934 and the Investment Company Act of 1940, this report has been signed below by the following persons on behalf of the registrant and in the capacities and on the dates indicated.

By: /s/ Keith F. Hartstein
-------------------------------------
Keith F. Hartstein
President and
Chief Executive Officer

Date: December 19, 2007

By: /s/ Charles A. Rizzo
-------------------------------------
Charles A. Rizzo
Chief Financial Officer

Date: December 19, 2007