Tagify™ off-the-shelf and custom loaded-transposase reagents provide reliability and scalability to tagmentation-based on- & off-target gene editing analysis

seqWell, a global provider of genomic library and multiplexing workflow solutions, today announced the launch of their new portfolio of Tagify loaded-transposase reagents, including Tagify i5 UMI adapter-loaded Tn5. In addition, seqWell will offer loading of transposases with customer-specified oligonucleotides, including loading of their high-performance TnX transposase.

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Tagify Adapter-loaded Transposase Reagents provide for reliable and scalable NGS-based on- and off-target gene editing analysis methods. (Photo: Business Wire)

The Tagify portfolio provides a commercial source of fully quality-controlled, loaded transposases with a defined level of enzyme activity and payload verification. This eliminates the need of laboratories to purify, load, and characterize the final loaded transposase prior to use in their assays, freeing up laboratory resources and reducing a source of assay-to-assay variability.

“Assays to define the genome-wide on- and off-target activity of genome editors are important for both biomedical research and therapeutics,” said Shengdar Tsai, Ph.D, Associate Member, Department of Hematology, St. Jude Children’s Research Hospital. “Broadly adopted, sensitive, unbiased, biochemical and cellular methods of understanding the genome-wide activity of editors, such as CHANGE-seq and GUIDE-seq-2, rely on transposase-based tools. The commercial availability of standardized transposase reagents will help lower barriers for more labs to implement these assays rapidly and reliably.”

Tagify i5 UMI adapter-loaded transposase reagents support gene editing analysis assays, such as the UdiTaS™ method, that rely on tagmentation to simultaneously fragment and tag genomic DNA with Illumina-compatible adapters using a highly scalable workflow. These off-the-shelf reagents are available as a set of either 24 or 96 transposases each containing an i5 barcode and a unique molecular indentifier (UMI) for high throughput applications.

The Tagify custom-loading option enables scientists to address the growing number of NGS-based on- and off-target gene editing methods that rely on tagmentation via the flexibility to load either hyperactive Tn5 or seqWell’s recently introduced high performance TnX transposase, with Illumina-compatible barcodes, UMIs, universal adaptors, or a custom oligonucleotide sequence.

“Our early access customers valued the availability of a reliable source of quality transposase-based reagents with their payload of choice, and Tagify products build on seqWell’s expertise in transposase loading and manufacturing to deliver these tools” said Joe Mellor, Chief Scientific Officer at seqWell. “Tagify i5 UMI is a great example of this capability, focused on scientists developing new genome editing methods and therapeutics. The high level of batch-to-batch consistency and availability of up to 96 UMIs will support the reproducible and scalable analysis of on- and off-target effects in sequencing-based genome editing QC assays.”

For more information about the use of Tagify loaded transposase reagents, view an on-demand webinar entitled, “Assessing CRISPR On-Target Editing and Structural Changes with UDiTaS™ using Tagify™ Reagents” presented by Georgia Giannoukos, Ph.D., Director of Next Generation Sequencing, at Editas Medicine.

About seqWell

seqWell creates scalable genomics technologies that simplify workflows and help enable every life scientist to unlock transformative discoveries with sequencing. seqWell offers a range of next-generation library prep products and workflows that allow customers to achieve higher levels of sample multiplexing quickly and easily. Learn more about us at www.seqwell.com or follow us on Linkedin.

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